We designed the part based on K541545. We found that R0010 might keep express genes below without regulation.Reason may be that Lacl expressed by E.coil is limited. So we inserted parts that express Lacl and constitutive promoter before the old one to inhibite pLac.
Notes
This part had not been test yet.
Source
The part was composed by parts provided by IGEM 2013 plants.