Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: CHANGXU WANG
Date created: 2014-09-27 11:00:00
Date modified: 2015-05-08 01:10:06
Anti-LPS factor(LALF) regulated by IPTG
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1363201_sequence (Version 1) |
Description
This part is a cell-kill system. Firstly,constitutive promoter could express enough Lacl that is useful for downstream. Secondly, the reporter in the middle of our part wouldn't show signal nor the cell be killed by the part behind unless IPTG or lactose added into the culture medium.No IPTG or lactose: GFP don't express ,G- won't be killed.
IPTG or lactose exsisting : GFPs express ,G- will be dead.
G-:Gram negative bacteria
Notes
This part had not been test yet.What we tested is that the lactose operon.We designed the part based on K541545. We found that R0010 might keep expressing genes downstream without regulation.Reason may be that Lacl protein which plays an important role in the lactose regulation process expressed by E.coli is limited. So we inserted parts which can expresses Lacl continuously in E.coli. into the origin one, so that we could strengthen the regulation by lactose.
Since we had got a wonderful gene switch controlled by lactose, we put a protein generator generating signal peptides and Limulus anti-LPS factors(LALF) downstream the switch. The signal peptides helped the LALF binding to E.coli.'s LPS layer,so that the growth of E.coil. would be limited.
In general, we construct our parts to realize the idea of starting the suicide process of E.coil. by adding lactose(or IPTG) into the culture medium.
Source
The part was composed by parts provided by IGEM 2013 plants.| Access | Instance | Definition |
| public | BBa_K737051 | BBa_K737051 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K737051 | 1,2298 | BBa_K737051 |