BBa_K1365001

BBa_K1365001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1365001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Sandra Mous

Date created: 2014-09-23 11:00:00

Date modified: 2015-05-08 01:10:07

NisB

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K1365001_sequence (Version 1)

Description

NisB dehydrates the serines and threonines of the nisin precursor peptide made by NisA. Together with the genes NisA, NisT, NisC, NisP, NisR and NisK it is responsible for producing the lantibiotic nisin in Lactococcus lactis.

Notes

Used primers:
forward - 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGATAAAAAGTTCATTTAA-3'
reverse - 5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATCATTTCATGTATTCTT-3'
to PCR NisB from the genome and add the BioBrick prefix and suffix.

Source

Obtained by PCR on the genome of Lactococcus lactis NZ9700, a nisin producing strain.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
start	1,3	start_codon, feature/start
stop	2980,2982	stop_codon, feature/stop
NisB	1,2982	CDS, feature/cds

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1365001/1

Member of these Collections

• iGEM Parts Registry