BBa_K1365005

BBa_K1365005 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1365005

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Sandra Mous

Date created: 2014-09-23 11:00:00

Date modified: 2015-05-08 01:10:07

NisP

Details

• Types: DnaRegion
• Roles: CDS; Coding

Description

NisP codes for a leader peptidase in Lactococcus lactis, to cut of the leaderpeptide after nisin has been transported out of the cell. Together with the genes NisA, NisB, NisT, NisC, NisR and NisK it is responsible for producing the lantibiotic nisin in L. lactis.

Notes

Used primers:
forward - 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAAAAAAATACTAGGTTT-3'
reverse - 5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATCAATTTTTAGTCTTTC-3'
to PCR NisP from the genome and add the BioBrick prefix and suffix.

Changed the start codon from a GTG to an ATG.

The illegal PstI sites at position 225 and the illegal EcoRI sites at postion 347 and 1657 were removed using primers as well, fusing the resulting PCR fragments together again with a Gibson assembly.

Source

Obtained by PCR on the genome of Lactococcus lactis NZ9700, a nisin producing strain.

Other Properties

• igem#sampleStatus: No part sequence
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1365005/1

Member of these Collections

• iGEM Parts Registry