BBa_K1365006

BBa_K1365006 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1365006

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Sandra Mous

Date created: 2014-09-23 11:00:00

Date modified: 2015-05-08 01:10:07

NisR and NisK

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K1365006_sequence (Version 1)

Description

Coding part for the genes NisR and NisK.

NisK is a membrane-associated protein kinase that phosphorylates NisR in response to enviromental signals. NisR is a regulatory protein, regulating PNisI and other promoters.

Together with the genes NisA, NisB, NisC, NisP they are responsible for producing the lantibiotic nisin in Lactococcus lactis.

Notes

Used primers:
forward - 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGTATAAAATTTTAATAGT-3'
reverse - 5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTACTTTTTTATTTTTA-3'
to PCR NisR and NisK from the genome and add the BioBrick prefix and suffix.

Changed the GTG start codon of NisR into a ATG.

Source

Obtained by PCR on the genome of Lactococcus lactis NZ9700, a nisin producing strain.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
start	1,1	start_codon, feature/start
start	680,680	start_codon, feature/start
stop	684,684	stop_codon, feature/stop
stop	2021,2021	feature/stop, stop_codon
NisR	1,686	feature/cds, CDS
NisK	680,2023	feature/cds, CDS

Other Properties

• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1365006/1

Member of these Collections

• iGEM Parts Registry