Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Rick Elbert
Date created: 2014-10-07 11:00:00
Date modified: 2015-05-08 01:10:07
anti Staphylococcus aureus Secretion system
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K1365100_sequence (Version 1) |
Description
Notes
RBS was assembled to BBa_K1365111 and BBa_I746104 with PCR. afterwhich all the parts were assembled with gibson assembly.Source
the P2 promotor and Double terminator came from the iGEM distribution kit 2014.NisA originates from the genomic sequence of L. lactis NZ9700.
DspB was synthesized by IDT.
| Sequence Annotation | Location | Component / Role(s) |
| I746104 start K1365111 (USP45-dspB) stop stop K1365000 (nisA) start stop B0015 B0010 B0012 B0034 B0034 | 1,95 121,121 121,1290 1285,1285 1288,1288 1316,1489 1316,1316 1487,1487 1497,1625 1498,1554 1592,1611 102,113 1297,1308 | promoter feature/promoter feature/start start_codon CDS feature/cds feature/stop stop_codon stop_codon feature/stop feature/cds CDS feature/start start_codon feature/stop stop_codon engineered_region feature/BioBrick stem_loop feature/stem_loop feature/stem_loop stem_loop feature/rbs ribosome_entry_site feature/rbs ribosome_entry_site |