BBa_K1366001

BBa_K1366001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1366001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Eduardo Cepeda Ca??edo

Date created: 2014-10-09 11:00:00

Date modified: 2015-05-08 01:10:07

Genetic construct for lpp gen deletion

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K1366001_sequence (Version 1)

Description

This biobrick contains the sequence homologies (50bp) to delete lpp gen (LPS moiety carrier) in E. coli with an ampicillin resistance and a Multiple Cloning Site (for the generation of genomic knock-ins of any desired gen in E. coli). The deletion of lpp gene and the replacement of that sequence with the Amp resistance. Gene deletions are performed with lambda-red recombination technology. FLP-FRT sequences are included to remove the antibiotic resistance with a specific recombinase.

Notes

This is the general structure
50bp homology Lpp // FRT // Amp R Promoter // RBS // AmpR // Terminator // FRT // MCS // 50 bp homology Lpp

Source

The genetic design is based in the lpp deletion done by:
Ni, Y., Reyes, J., & Chen, R. (2007). lpp Deletion as a Permeabilization Method. Biotechnology and Bioengineering, 1347-1356.
It also contains some parts of the registry like BBa_K1073000, BBa_B0034, BBa_B0015

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1366001/1

Member of these Collections

• iGEM Parts Registry