Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Eduardo Cepeda Ca??edo, Mercedes Alejandra Vazquez Cantu
Date created: 2014-10-09 11:00:00
Date modified: 2015-05-08 01:10:07
GFP expression under a hTERT promoter (C4)
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1366103_sequence (Version 1) |
Description
This composite act as a probe of concept of the transfection in mammalian cells mediated by bacteriophages. It contains the hTERT promoter, specific for cancerous cells lines. The construct will be contained in a phagemid with de f1 origin of replication (for single stranded DNA), and it is intended to replace the M13 genome. The GFP will be codon optimized for mammalian cell line.Notes
The structure goes like this hTERT // RBS // GFP // TerminadorSource
We obtain the sequence of the hTERT promoter from the part BBa_K404106 and we used the GFP sequence that is available in uniprot and optimized it for mammalian cell expression| Sequence Annotation | Location | Component / Role(s) |
| phTERT promoter BBa_K404106 hGH terminator BBa_K404108 Kozak sequence GFP | 1,459 1,459 1185,1666 1185,1666 460,472 473,1184 | feature/promoter promoter engineered_region feature/BioBrick sequence_feature feature/misc engineered_region feature/BioBrick ribosome_entry_site feature/rbs feature/cds CDS |