C6

BBa_K1366105 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1366105

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Eduardo Cepeda Ca??edo, Mercedes Alejandra Vazquez Cantu

Date created: 2014-10-09 11:00:00

Date modified: 2015-05-08 01:10:07

Production of survivin siRNA under the hTERT promoter (C6)

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K1366105_sequence (Version 1)

Description

This construct will be used to characterize the transfection of an effector of death (Survivin siRNA) in mammalian cells mediated by bacteriophages. It contains the hTERT promoter, specific for cancerous cells lines. The construct will be contained in a phagemid with de f1 origin of replication (for single stranded DNA), and it is intended to replace the M13 genome.

Notes

The design sequence is as follows: hTERT // siRNA // terminador

Source

We designed the siRNA. And the other parts come from the iGEM registry

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
hTERT promoter	1,459	promoter, feature/promoter
survivin siRNA 1	460,513	feature/dna, sequence_feature
hGH polyadenylation	514,995	feature/stop, stop_codon

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1366105/1

Member of these Collections

• iGEM Parts Registry