Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Eduardo Cepeda Ca??edo, Mercedes Alejandra Vazquez Cantu
Date created: 2014-10-09 11:00:00
Date modified: 2015-05-08 01:10:07
Production of survivin siRNA under a hTERT promoter (C7)
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1366106_sequence (Version 1) |
Description
This construct will be used to characterize the transfection of an effector of death (Survivin siRNA) in mammalian cells mediated by bacteriophages. It contains the hTERT promoter, specific for cancerous cells lines. The construct will be contained in a phagemid with de f1 origin of replication (for single stranded DNA), and it is intended to replace the M13 genome.Notes
The general structure is as follows hTERT // siRNA // terminadorSource
We design our own survivin siRNA and for the other parts we used the Registry parts| Sequence Annotation | Location | Component / Role(s) |
| hTERT promoter hGH polyadenylation survivin siRNA 2 | 1,459 510,991 460,509 | promoter feature/promoter feature/misc sequence_feature sequence_feature feature/dna |