BBa_K1369002

BBa_K1369002 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1369002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Andrew Hou

Date created: 2014-08-17 11:00:00

Date modified: 2015-05-08 01:10:08

Activator-Repressor promoter (AraC and TetR operator sites)

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K1369002_sequence (Version 1)

Description

This is a repressor-repressor (RR) combinatorial promoter which consists of a LacI operator site upstream of the -35 RNA polymerase binding site, and a TetR operator site within the -35 and -10 RNA polymerase boxes.

Notes

To test the logic of this RR promoter, we assembled constructs containing the promoter, a bicistronic RBS (part BBa_J119024), and GFP, in that order, downstream of the promoter.

Source

This part is taken from the paper "Programming gene expression with combinatorial promoters," by Robert Sidney Cox III et al.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
AraC binding site	1,43	feature/operator, operator
-35 RNA polymerase box	40,45	feature/misc, sequence_feature
TetR binding site	45,67	feature/operator, operator
-10 RNA polymerase box	63,68	sequence_feature, feature/misc
TetR binding site	70,103	feature/operator, operator

Other Properties

• igem#experience: Works
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1369002/1

Member of these Collections

• iGEM Parts Registry