BBa_K1369003

BBa_K1369003 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1369003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Andrew Hou

Date created: 2014-09-03 11:00:00

Date modified: 2015-05-08 01:10:08

AgrC+4xSH3 domain

Details

• Types: DnaRegion
• Roles: Signalling; engineered_region
• Sequences: BBa_K1369003_sequence (Version 1)

Description

This sequence encodes the AgrC histidine kinase with a 4xSH3 scaffold domain attached at the C-terminus.

Notes

AgrC is a transmembrane histidine kinase. Because the 4xSH3 domain is part of a scaffold system that increases binding affinity between AgrC and the response regulator AgrA, it is necessary that the 4xSH3 domain be attached to the C-terminus, which faces the inside of the cell.

Source

AgrC is endogenous to Staphylococcus aureus, and is a histidine kinase receptor involved in the AgrBDCA quorum sensing system.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
pCon	1,29	feature/promoter, promoter
RBS	30,73	feature/rbs, ribosome_entry_site
AgrC	74,1315	CDS, feature/protein
GS-linker	1316,1345	sequence_feature, feature/misc
GS-linker	1346,1381	sequence_feature, feature/misc
SH3 (1)	1381,1554	feature/misc, sequence_feature
GS-linker	1555,1590	feature/misc, sequence_feature
SH3 (2)	1591,1764	feature/misc, sequence_feature
GS-linker	1765,1800	feature/misc, sequence_feature
SH3 (3)	1801,1974	feature/misc, sequence_feature
GS-linker	1975,2010	sequence_feature, feature/misc
SH3 (4)	2011,2184	sequence_feature, feature/misc
stop codon	2185,2205	stop_codon, feature/stop

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1369003/1

Member of these Collections

• iGEM Parts Registry