BBa_K137014

BBa_K137014 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K137014

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Doug Tischer

Date created: 2008-06-19 11:00:00

Date modified: 2015-05-08 01:10:08

katE +LAA

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K137014_sequence (Version 1)

Description

katE (catalase) from E. coli. Site directed mutagensis to remove an EcoRI site, and an added C-term ssrA (LAA) tag for fast cell clearance.

Notes

Removal of an EcoRI site by site directed mutagenesis and addition of an ssrA tag with PCR primers

Source

E. coli genomic PCR and addition of ssrA tag with primers during PCR

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
C->T	1041,1041	sequence_alteration, feature/mutation
katE	1,2298	CDS, feature/cds
LAA	2260,2292	tag, feature/tag

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K137014/1

Member of these Collections

• //function/degradation
• iGEM Parts Registry