BBa_K137016

BBa_K137016 Version 1

Component

This part has been discontinued.

Source: http://parts.igem.org/Part:BBa_K137016

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Allen Lin

Date created: 2008-07-09 11:00:00

Date modified: 2015-05-08 01:10:08

GFP with (AC)21 repeat after start codon

Details

• Types: DnaRegion
• Roles: Reporter; engineered_region
• Sequences: BBa_K137016_sequence (Version 1)

Description

ATG AC ACACACACAC ACACACACAC ACACACACAC ACACACACAC cgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtg
aaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatgg
tgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttc
aaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatg
gaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaat
tagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattac
ctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatgg
atgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtga
acgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata

Notes

The number of AC repeats was limited by maximation length of synthesized primers.

Source

The dinucleotide repeat was placed into synthesized primers that bound to the existing part I13401, and then a PCR reaction was run.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
ATG	1,1	start_codon, feature/start
AC repeat	4,45	sequence_feature, feature/misc

Other Properties

• igem#experience: None
• igem#sampleStatus: Discontinued
• igem#status: Deleted
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K137016/1

Member of these Collections

• iGEM Parts Registry