BBa_K137023

BBa_K137023 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K137023
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Allen Lin
Date created: 2008-07-18 11:00:00
Date modified: 2015-05-08 01:10:09

GFP with (A)23 repeat after start codon



    • Details

    Types
    DnaRegion

    Roles
    engineered_region

    Reporter

    Sequences BBa_K137023_sequence (Version 1)

    Description

    GFP with (A)23 repeat after start codon

    Notes

    The nucleotide repeat was placed into synthesized primers that bound to the existing part I13401, and then a PCR reaction was run.

    Source

    With 23 A repeats, the GFP sequence is -1 frame from the start codon, and so GFP should not be transcribed.

    Sequence Annotation Location Component / Role(s)
    ATG
    A repeat
    		1,1
    4,26
    		start_codon feature/start
    feature/misc sequence_feature

    igem#experience
    Issues
     
    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K137023/1
     

    Attachments

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