Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Allen Lin
Date created: 2008-07-19 11:00:00
Date modified: 2015-05-08 01:10:09
GFP with (CTTCT)8 repeat after start codon
| Types | DnaRegion |
| Roles | Reporter engineered_region |
| Sequences | BBa_K137026_sequence (Version 1) |
Description
GFP with (CTTCT)8 repeat after start codonNotes
With 8 CTTCT repeats, the GFP sequence is in the +1 frame with respect to the start codon, so GFP should not be transcribed.The number of CTTCT repeats was limited by maximum length of synthesized primers.
Source
The pentanucleotide repeat was placed into synthesized primers that bound to the existing part I13401, and then a PCR reaction was run.| Sequence Annotation | Location | Component / Role(s) |
| ATG CTTCT repeat | 1,1 4,43 | feature/start start_codon feature/misc sequence_feature |