Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Allen Lin
Date created: 2008-07-20 11:00:00
Date modified: 2015-05-08 01:10:09
250 bp inverted tetR promoter
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_K137047_sequence (Version 1) |
Description
Inverted tetR promoter with noncoding, spacer DNA downstream of it to make the total length 250 bp. The promoter faces the 5' direction.Notes
This part is in a series of inverted tetR promoters that have total lengths of 150, 250, 350, 450, 650, and 850 bp. We chose the region upstream of tetR in part Q04400 to be the noncoding DNA segment. This noncoding segment consists of the 3' end of tetR and B0015. None of the parts in this series was long enough to include the start codon of tetR, so a functional tetR protein should not be transcribed.Source
Primers were synthesized to bind to part Q04400, and then a PCR reaction was run.| Sequence Annotation | Location | Component / Role(s) |
| tetR promoter | 1,54 | promoter feature/promoter |