| Types | DnaRegion
|
| Roles | CDS
Coding
|
| Sequences | BBa_K1378031_sequence (Version 1)
|
Description
Holin is a 105-amino-acid-residue cytoplasmic membrane protein with three transmembrane domains, naturally expressed by double-stranded lambada phage. Holin will oligomerize and form a hole on the inner membrane of host bacteria at a certain time at an allele-specific time. And then the formation of hole will help Endolysin, a kind of lysozyme, come out from cytoplasm to periplasm to degrade peptidoglycan and inhibit the respiration by eliminating proton gradient.
Notes
Because Holin will do harm to bacteria, we need to carefully inhibi it's expression if we put the gene of Holin into the bacteria.
Source
We get this part by de novo synthesis.
