BBa_K1391012

BBa_K1391012 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1391012
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Shinjini Saha
Date created: 2014-10-16 11:00:00
Date modified: 2015-05-08 01:10:14

pENTR_Cofilin (Inactivated - pseudophosphorylated)



    • Details

    Types
    DnaRegion

    Roles
    plasmid

    Plasmid

    Sequences BBa_K1391012_sequence (Version 1)

    Description

    Cofilin is an actin depolymerizing factor. It is recruited when PirB/LilrB2 is activated by beta-amyloid. [1]

    The 3rd amino acid in this construct has been mutated to make it pseudophophoralated and inactive. It was ordered from Adgene.

    [1] Taeho Kim, George S. Vidal, Maya Djurisic, et al. "Human LilrB2 is a beta-amyloid recptor and its murine homolog PirB regulates synaptic plasticity in an Alzheimer's Model". Science. 20 Sep 2013. . 16 Oct 2014.

    Notes

    We did not want cofilin to interfere with the normal metabolism of the cell, so we made it pseudophosphorylated, hence inactive.

    Source

    Human

    igem#experience
    None
     
    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K1391012/1
     

    Attachments

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