| Types | DnaRegion
|
| Roles | Plasmid
plasmid
|
| Sequences | BBa_K1391135_sequence (Version 1)
|
Description
This is vector expresses a miRNA to down-regulate BACE1 expression
Notes
There are a variety of factors to consider when designing a miRNA to effectively down-regulate a gene of interest. Among these are the region within the gene that the miRNA binds, the degree of complementarity between the miRNA and its target sequence, the bases flanking the mature miRNA sequence, and non-specific down-regulation of other undesirable targets. To optimize these parameters we referred to online tools such as Ohio State University???s miR-Synth tool.
Source
The miRNA sequence was assembled via Golden-Gate with two G-blocks. The miRNA sequence was then assembled with the promoter part pENTR TRE by Gateway.
