| Types | DnaRegion
|
| Roles | Plasmid
plasmid
|
| Sequences | BBa_K1391139_sequence (Version 1)
|
Description
This vector expresses BACE1 tagged with eYFP (5???-BACE1-eYFP-3???)
Notes
In designing BACE1 and BACE2 tagged with eYFP, we considered the possibility that linking eYFP to these enzymes might inhibit their function or expression. With this in mind, for both BACE-eYFP and BACE2-eYFP we designed two variants???one with eYFP linked to the 5??? end, and another with eYFP linked to the 3??? end.
Source
This vector was assembled by linking BACE1 and YFP via Golden Gate. BACE1 was purchased commercially.
