Types | DnaRegion
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Roles | Composite
engineered_region
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Sequences | BBa_K1392935_sequence (Version 1)
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Description
This part contains the promoter and its coding sequence for the repressor AraC This is transcribed in upstream direction by binding to L(+)-arabinose, AraC changes its conformation.
Notes
We could easily use this part by binding it with a RBS, to produce an inducible regulatory promoter/repressor unit.
Source
In Escherichia coli, the AraC protein represses transcription from its own promoter, PC, and when it is associated with arabinose, it activates transcription from three other promoters, PBAD, PE, and PFGH. Expression from all four of these promoters is also regulated by cyclic AMP-catabolite activator protein; however, the arrangement of the protein binding sites is not identical for each promoter.