BBa_K1392938

BBa_K1392938 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1392938
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Şeniz Y??ksel Burak Kızıl Helin Tercan
Date created: 2014-10-06 11:00:00
Date modified: 2015-05-08 01:10:15

araC-Pbad + RBS + tetR- LVA



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K1392938_sequence (Version 1)

Description

This part is a composite structure which contains a promoter, RBS side and and a tetracycline repressor from transposon Tn10. It is transcribed in the opposite direction for the coding sequence of the repressor AraC. Through the binding to L+arabinose, the conformation of AraC changes.This causes the protein to diffuses from the DNA thereby inducing transcription. Through the RBS, the definition of the RBS efficiency could be observed.

Notes

L(+) - Arabinose is in sugar form and harmless
The designed promoter has low background activity, so the membrane proteins will not damage the cells before induction.

Source

References:
//parts.igem
Schleif, R. (2000). "Regulation of the L-arabinose operon of Escherichia coli." Trends Genet 16(12): 559-565.
Ren, H., D. Yu, et al. (2009). "High-level production, solubilization and purification of synthetic human GPCR chemokine receptors CCR5, CCR3, CXCR4 and CX3CR1
http://openwetware.org/wiki/Titratable_control_of_pBAD_and_lac_promoters_in_individual_E._coli_cells#pBAD_promotersOpenWetWare
http://www.ncbi.nlm.nih.gov/pubmed/7768852?dopt=Abstract

Access Instance Definition
public
public
public
BBa_K808000
BBa_B0034
BBa_C0040
Ara
BBa_B0034
tetR
igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1392938/1