| Types | DnaRegion
|
| Roles | engineered_region
Composite
|
| Sequences | BBa_K1392939_sequence (Version 1)
|
Description
This part is a composite structure which contains a RBS side and and a tetracycline repressor from transposon Tn10. Through the RBS, the definition of the RBS efficiency could be observed. It is a coding region for the TetR protein with the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. TetR binds to the pTet regulator (Part:BBa_R0040). aTc (anhydrotetracycline) binds to TetR and inhibits its operation.
Notes
It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
Source
pTet regulator (Part:BBa_R0040)
