BBa_K1392970

BBa_K1392970 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1392970

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Şeniz Y??ksel

Date created: 2014-10-09 11:00:00

Date modified: 2015-05-08 01:10:15

Terminator+Tetr Promoter+T4 Endolysin

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K1392970_sequence (Version 1)

Description

For this part an artifical Terminator is used (generally added to another parts). The promoter is a TetR repressible promoter.It is sequenced for pTet inverting regulator. Promoter is constitutively ON and repressed by TetR. TetR repression is inhibited by the addition of tetracycline or its analog part

Notes

Bidirectional, with the reverse estimated to be more effective than the forward. Has a polyA tail of 9 residues.
High expression of lysozyme only is toxic to bacteria.
BBa_R0040 (TetR repressible promoter) is based on a cI promoter. It has been modified to include two TetR binding sites.
Constructed by DNA synthesis.

Source

Enterobacteria phage T4 http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=29366675&from=66503&to=66997&view=gbwithparts
modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs
BiblioPlus Extension Error fetching PMID 9092630:

Components

Access	Instance	Definition
public	BBa_B1006	BBa_B1006
public	BBa_R0040	p(tetR)
public	BBa_K112806	BBa_K112806

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_B1006	1,39	BBa_B1006
BBa_R0040	48,101	p(tetR)
BBa_K112806	110,623	BBa_K112806

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1392970/1

Member of these Collections

• //direction/forward
• //terminator
• iGEM Parts Registry