BBa_K1393000

BBa_K1393000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1393000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jiajun Tan
Date created: 2014-10-01 11:00:00
Date modified: 2015-07-22 02:47:15

OprF(Val188)+GS linker+CBP



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K1393000_sequence (Version 1)

Description

The OprF is a major outer membrane protein of Pseudomonas aeruginosa. This protein functions as a nonspecific porin to allow the passage of small hydrophilic molecules, plays a structural role in maintaining cell shape and outer membrane integrity, and is required for growth under low osmolality.The structure of OprF has been proposed to consist of three domains, the N-terminal forming h-barrel structure, a loop or hinge region, and the C-terminal associated with peptidoglycan.

Notes

Based on the predicted secondary structure and information found in the literature, we chose Val(188)as potential fusion sites for displaying CBP.CBP is the abbreviation of copper binding peptide made up of seven amino acid.In order to reduce the effect on the CBP activity,we added (G4S) linker between OprF and CBP .

Source

The OprF gene is from Pseudomonas aeruginosa.

Sequence Annotation Location Component / Role(s)
OprF to Val188
CBP
1,564
579,600
feature/protein CDS
non_covalent_binding_site feature/binding
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BBa_K1393000/1