| Types | DnaRegion
|
| Roles | CDS
Coding
|
| Sequences | BBa_K1396002_sequence (Version 1)
|
Description
The part is an coding sequence for an anti-microbial peptides linked to a mussel-foot protein-linked to superfolder GFP for localization. The mussel foot protein will anneal to surfaces as a wet glue and superfolder GFP will allow for florescence imaging and localization. In order to use this part you can produce it in a TAG recoded organism simultaneously expressing a Tyrosine supressor or L-DOPA orthogonal translational system. In order to purify you can use the 2X Strep tag and strep column and later cleave with enterokinase to remove the sequence supressing LL-37 antimicrobial action.
Notes
There are linker units between individual components of DNA and restriction sites built for modularity.
Source
Synthesized in Lab
