Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Anna Stikane
Date created: 2014-09-18 11:00:00
Date modified: 2015-05-08 01:10:16
Ptet-GFP(DAS)
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K1399023_sequence (Version 1) |
Description
Tetracycline/aTc inducible promoter with GFP reporter tagged with DAS-ssrA degradation tag followed by terminator.The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.Presence of SspB is crucial for effective protein degradation.
Notes
Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]Source
other biobricks| Access | Instance | Definition |
| public public public public | BBa_R0040 BBa_B0034 BBa_K1399008 BBa_B0015 | p(tetR) BBa_B0034 BBa_K1399008 BBa_B0015 |