Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Dylan Siriwardena
Date created: 2014-10-05 11:00:00
Date modified: 2015-05-08 01:10:16
PTRE(4)GX Dual input promoter. Activation at tetO binding sites, repression at gal4 sites.
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_K1400000_sequence (Version 1) |
Description
This is a dual input promoter that can be activated and repressed by two different activating proteens. is based off the Gal promoter in Saccharomyces cerevisiae, which is a relatively strong promoter. with four Tetr sites upstream of the TATA box. These can be bound by the tet repressor, or by activating variants using the Tetr binding domain like rtTA (tet binding domain, vp16 activating domain). 10bp downstream of the TATA box two Gal4 binding sites are added, which can be bound by Gal4 binding proteins or phusion activators like GEV (Gal4 binding domain, human estrogen receptor, vp16). The close proximity of these sites to the TATA box cause any binding protein (activating or repressing) to repress expression. The close proximity of these sites all seem to affect transcription rates of this promoter, as it has significantly less expression than the native Gal promoter. Thus this promoter can be used for complex control of expression.Notes
The major design consideration was the proximity of the repressing sites to the TATA box. 10bp seemed to cause strong repression while still not interfering too much with transcription when no proteins are bound.Source
This part was generated by modifying the native Gal promoter from Saccharomyces cerevisiae.| Sequence Annotation | Location | Component / Role(s) |
| Tet Binding site Tet Binding site Tet Binding Site Tet Binding site TATA box Gal4 Binding site Gal4 Binding site | 2,19 67,85 151,169 234,252 296,302 313,329 331,347 | feature/binding non_covalent_binding_site feature/binding non_covalent_binding_site feature/binding non_covalent_binding_site feature/binding non_covalent_binding_site feature/binding non_covalent_binding_site feature/binding non_covalent_binding_site feature/binding non_covalent_binding_site |