Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Dylan Siriwardena
Date created: 2014-10-05 11:00:00
Date modified: 2015-05-08 01:10:16
PGAL Modified native pGAL
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_K1400002_sequence (Version 1) |
Description
A lower expression pGAL promoter with 4 gal4 sites. This is used to have similar expression as pGALTX and PTREGX. It is a single input promoter, with scrambled mig1 sites.Notes
In the scrambling after the TATA box, the same GC content was maintained as with tet binding sites. Mig1 sites were scrambled to allow for grow up in glucose.Source
This part was generated by modifying the native Gal promoter from Saccharomyces cerevisiae.| Sequence Annotation | Location | Component / Role(s) |
| GAL4 binding site GAL4 binding site GAL4 binding site GAL4 binding site TATA box | 7,23 26,42 44,60 108,124 334,340 | operator feature/operator feature/operator operator operator feature/operator operator feature/operator sequence_feature feature/misc |