BBa_K1400003

BBa_K1400003 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1400003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Dylan Siriwardena

Date created: 2014-10-05 11:00:00

Date modified: 2015-05-08 01:10:16

pTre(4) Single input tet responsive promoter

Details

• Types: DnaRegion
• Roles: Regulatory; promoter
• Sequences: BBa_K1400003_sequence (Version 1)

Description

A single tet responsive promoter created by replacing the first 4 gal4 sites in the gal promoter with tet sites. It was designed to have similar expression to our modified gal promoter (BBA_K1400002).

Notes

Mig1 sites were scrambled to allow grow up in glucose.

Source

This part was generated by modifying the native Gal promoter from Saccharomyces cerevisiae.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
tetO	1,19	operator, feature/operator
tetO	151,169	operator, feature/operator
tetO	67,85	feature/operator, operator
tetO	234,252	sequence_feature, feature/misc
TATA box	296,302	feature/misc, sequence_feature

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1400003/1

Member of these Collections

• iGEM Parts Registry