BBa_K1403006

BBa_K1403006 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1403006
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Antonio Villarreal Larrauri
Date created: 2014-10-05 11:00:00
Date modified: 2015-05-08 01:10:17

Jasmonic acid carboxyl methyltransferase (JMT) expression cassette



    • Details

    Types
    DnaRegion

    Roles
    Generator

    engineered_region

    Sequences BBa_K1403006_sequence (Version 1)

    Description

    Jasmonic acid carboxyl methyltransferase coding sequence under constitutive promoter.

    Notes

    Silently removed an illegal PstI site from the codon optimised sequence. Added a C-terminus His tag.

    Source

    CDS of JMT (AY008434.1), codon optimised for E. coli.

    Sequence Annotation Location Component / Role(s)
    Promoter
    sRBS
    JMT
    -35
    -10
    Scar
    6-His
    Stop
    		1,35
    42,76
    77,1244
    1,6
    24,29
    36,41
    1244,1261
    1262,1264
    		feature/promoter promoter
    ribosome_entry_site feature/rbs
    CDS feature/cds
    feature/operator operator
    feature/operator operator
    feature/scar
    tag feature/tag
    feature/stop stop_codon

    igem#experience
    None
     
    igem#sampleStatus
    It's complicated
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K1403006/1
     

    Attachments

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