Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sierra Tackett
Date created: 2014-10-08 11:00:00
Date modified: 2015-05-08 01:10:17
pBAD promoter + strong RBS
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1406000_sequence (Version 1) |
Description
A combinatorial part of K206000, B0034, and a NheI cutsite. This allows a in-frame, orientated insertion of a biobrick part behind the pBAD promoter and the strong RBS using the restriction enzymes NheI and PstI.Notes
We say we are hoping to make a high-expressing, directional insertion system in which we used enzymes which were compatible with the BioBrick standard and as an end product form a BioBrick.Source
E. coli| Sequence Annotation | Location | Component / Role(s) |
| promoter AraI1 AraI2 BBa_K206000 conserved BBa_B0034 | 1,131 40,57 61,78 1,130 143,146 139,150 | promoter feature/promoter feature/operator operator feature/operator operator feature/BioBrick engineered_region conserved_region feature/conserved feature/BioBrick engineered_region |