BBa_K1412007

BBa_K1412007 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1412007
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Xiaotong Fu
Date created: 2014-08-30 11:00:00
Date modified: 2015-05-08 01:10:18

CheZ-TT



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_K1412007_sequence (Version 1)

Description

This part consists of a cheZ coding region which is responsible for the dephosphorylation of CheY protein in bacteria flagella movement. This part does not pose any biological threat. With a promoter of your interest, this device rescues the mobility of cheZ-/- cells. It has been reported that cheZ-/- strain has a higher frequency of direction change and thus a narrower range of mobility.
This part also has a RBS coding region whose efficiency is 1.0.

Biology


Chemotaxis in E. coli is well documented. These bacteria can perform two types of movement, tumbling and smooth swimming. The difference between the two is determined by flagellar movement. During tumbling movement, the flagella move clockwise. This is caused by the formation of a complex between CheY-P and FliM, one of the flagella-associated proteins. During smooth swimming, the flagella move counter-clockwise. CheY is not phosphorylated and therefore cannot associate with flagellar proteins, causing the flagella to rotate in the opposite direction.
Smooth swimming is the movement performed by bacteria towards an attractant or away from a repellent. Smooth swimming is controlled by a number of chemotaxis proteins that make up a signalling pathway, please see the demonstration below for details.

Usage


We performed a swarming assay to characterize the motility of cheZ-deficient strain CL1 and cells transformed with cheZ-expressing plasmid.(BBa_K1412001).

Our results clearly show that cheZ-deficient mutants show smaller diffusion, while they are rescued by our BBa_K1412001 plasmid.

[[Image:IMG_20140825_185642.jpg|300px]];


Reference


[1] Paungfoo-Lonhienne C et al. (2010) Turning the table: plants consume microbes as a source of nutrients. PLoS One 5(7): e11915.

[2] Chelsky D and Dahlquist FW (1980) Chemotaxis in Escherichia coli: association of protein components. Biochemistry 19: 4633???4639

Notes



Source



Access Instance Definition
public
public
BBa_K629003
BBa_B0015
cheZ
BBa_B0015
Sequence Annotation Location Component / Role(s)
BBa_K629003
BBa_B0015
1,645
654,782
cheZ
BBa_B0015
igem#experience
None
 
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1412007/1