This part is composed to OriVR6Kgamma, Tn10 and 2 sites Is10. Between Is10 there are sites EcoRI XbaI SpeI and PstI. This is a Universal Transposon Plasmid.
Notes
This plasmid has been built by Golden Gate
Source
Each blend provide to plasmid pNK2. This is Bryan JESTER who give pNK2.