BBa_K1421000

BBa_K1421000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1421000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Mengni Wang

Date created: 2014-09-22 11:00:00

Date modified: 2015-05-08 01:10:22

plux R+RBS+LacIM1+2TE

Details

• Types: DnaRegion
• Roles: engineered_region; Device

Description

This device is comprised of lux pR (BBa_R0062), RBS (BBa_B0034), lacIM1 (BBa_K082005) and 2 TE (BBa_B0010 and BBa_B0012).
The lux pR is a right promoter in the lux cassette of V. Fischeri, which gives weak constitutive expression while enhanced when binding to the complex of LuxR and AHL (acyl-homoserine lactone ).
The LacIM1 is a LacI mutant, which can repress lac promoter. Thanks to the work of Tokyo_Tech 2010, it is confirmed that this mutant protein has a weaker function of repression to the lac promoter, compared to the wild type LacI.

Notes

Because it comes from several parts' combination, we tried to connect them together in a more convenient way.

Source

This device is a combination of lux pR (BBa_R0062), RBS (BBa_B0034), lacIM1 (BBa_K082005) and 2 TE (BBa_B0010 and BBa_B0012).

Other Properties

• igem#sampleStatus: No part sequence
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1421000/1

Member of these Collections

• iGEM Parts Registry