BBa_K1423005

BBa_K1423005 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1423005

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Shawna Henry

Date created: 2014-09-22 11:00:00

Date modified: 2015-05-08 01:10:23

Const. Promoter-RBS-BclA-YFP-DT

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K1423005_sequence (Version 1)

Description

This is a full construct including a constitutive promoter and RBS to maximize the expression of YFP which is localized to the cell surface using BclA.

Notes

During the design of this multiple domain sequence, we needed to ensure that all domains remained in the same reading frame.

Source

BclA is from bacillus anthracis while YFP is a mutant GFP from the jellyfish Aequorea victoria.

Components

Access	Instance	Definition
public	BBa_J23100	BBa_J23100
public	BBa_B0034	BBa_B0034
public	BBa_K1423003	BBa_K1423003
public	BBa_B0015	BBa_B0015

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_J23100	1,35	BBa_J23100
BBa_B0034	44,55	BBa_B0034
BBa_K1423003	64,855	BBa_K1423003
BBa_B0015	864,992	BBa_B0015

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1423005/1

Member of these Collections

• iGEM Parts Registry