| Types | DnaRegion
|
| Roles | RNA
mature_transcript_region
|
| Sequences | BBa_K1427000_sequence (Version 1)
|
Description
The merRNA (massively expressed regulatory RNA) is a long, non-coding RNA transcript found in Bdellovibrio bacteriovorus which was described by Karnuker et al 2013. The 445 nt transcript contains a cyclic-di-GMP riboswitch sequence and is believed to mediate a change from growth phase to attack phase in Bdellovibrio by sequestering the second messenger c-di-GMP. When expressed constructively under a T7 promoter in K12 E. coli, the merRNA increased biofilm formation. This is thought to be the result of the merRNA binding to and sequestering c-di-GMP molecules. This causes an increase in motility and flagella expression. The additional flagella adhere to surfaces and other cells, thereby forming a robust biofilm. Teams can insert this part directly downstream of a promoter sequence to promote stronger biofilm formation.
Notes
Teams should be mindful of their transcriptional start site when using the merRNA. The promoter should be constructed such that no additional nucleotides will be transcribed before the merRNA sequence, as this may cause misfolding.
Source
Bdellovibrio bacteriovorus
