Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Cedric Bl??tz
Date created: 2014-09-29 11:00:00
Date modified: 2015-05-08 01:10:23
yeast LEU2 gene
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1430001_sequence (Version 1) |
Description
What does the marker help synthesize?L-leucine
Where it can be used?
In LEU2 deficient strains of S. cerevisiae
Can it be used in different yeasts?
yes - This can complement leu1- S. pombe, but mulitple copies are required.
Notes
We mutated the restriction site for EcoR1 in the sequence that is gets compatibel to Prefix/Suffix enzymes. We used PCR approch with primers that add a different base in the recognition site.Source
genomic DNA of S. cerevisiae| Sequence Annotation | Location | Component / Role(s) |
| LEU2 s_mutation | 1,1095 639,639 | feature/protein CDS point_mutation feature/s_mutation |