RBS-GsiB

BBa_K143020 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K143020
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: James Chappell
Date created: 2008-09-14 11:00:00
Date modified: 2015-05-08 01:10:23

GsiB ribosome binding site (RBS) for B. subtilis



    • Details

    Types
    DnaRegion

    Roles
    ribosome_entry_site

    RBS

    Sequences BBa_K143020_sequence (Version 1)

    Description

    GsiB is an endogenous ribosome binding site from B.subtilis. The sequence of the gsiB ribosome binding site is AAAGGAGG which is complementary to the sequence UUUCCUCC from the 3' region of the 16s rRNA from B.subtilis.
    GsiB is an endogenous ribosome binding site (RBS) from B.subtilis. The sequence of the gsiB ribosome binding site is AAAGGAGG which is complementary to the sequence UUUCCUCC from the 3' region of the 16s rRNA from B.subtilis. Previous research showed that the predicted binding energy of the 16s rRNA to the RBS is -9.3kcal.

    Notes

    In order to ensure that the RBS is functional the actual ribosome binding site was maintained and the distance between the RBS and the start codon maintained. In order to conform to the biobrick standard the sequence flanking the RBS had to be changed but the distance between the promoter and RBS, and start codon and RBS was maintained.

    Source

    The sequence was taken from a previous research paper [1] and was constructed by Geneart

    Sequence Annotation Location Component / Role(s)
    rbs
    		2,8
    		feature/rbs ribosome_entry_site

    igem#experience
    None
     
    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K143020/1
     

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