BBa_K143075

BBa_K143075 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K143075

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Chris Hirst

Date created: 2008-10-27 12:00:00

Date modified: 2015-05-08 01:10:24

B. subtilis AmyE locus GFP output promoter and RBS tester

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K143075_sequence (Version 1)

Description

AmyE 3' Integration sequence(BBa_K143002) coupled to GFP proein coding region and the registry double terminator (part BBa_I13401.

The amyE 3' integration sequence allows integration into the B. subtilis genome at the amyE locus if the 5' amyE integration sequence(BBa_K143001) is cloned onto the 5' end of the construct.

The GFP coding region gives a fluorescent output when a promoter and RBS are placed immediately upstream of the coding region while the terminator prevents readthrough.

Notes

The amyE 3' integration sequence was PCR cloned from the B. subtilis integration vector pDR111 utilising Pfu DNA polymerase. GFP and the double terminator were obtained from the registry.

Source

The amyE 3' integration sequence was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase and cloned into a BioBrick with the GFP and the double terminator that were obtained from the registry.

Components

Access	Instance	Definition
public	BBa_I13401	BBa_I13401
public	BBa_K143002	amyE 3 IS

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_I13401	1,857	BBa_I13401
BBa_K143002	866,1867	amyE 3 IS

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K143075/1

Member of these Collections

• //chassis/prokaryote/bsubtilis
• iGEM Parts Registry