BBa_K1433002

BBa_K1433002 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1433002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Hanqing Liu

Date created: 2014-09-27 11:00:00

Date modified: 2015-05-08 01:10:25

attL-J23110-attR

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K1433002_sequence (Version 1)

Description

Bxb1 gp35 is a serine integrase and Bxb1 gp47 is an excisionase in Mycobacterium phage Bxb1.
This part is composed of 2 elements.
1. Promoter (reverse): BBa_J23110, a middle-ground Bacterial constitutive promoter.
2. attL and attR sites: Recognition site for Bxb1 gp35, Mycobacterium Phage Bxb1 DNA integrase.
This part promotes transcription and translation upstream gene, and when treat with heterologous of Bxb1 gp35 and gp47, downstream gene will express.

Notes

No

Source

this parts are construct from BBa_J23110 by adding attL/attR sites using PCR

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
attL	1,50	feature/dna, sequence_feature
J23110	51,85	feature/promoter, promoter
attR	86,138	sequence_feature, feature/dna

Other Properties

• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1433002/1

Member of these Collections

• iGEM Parts Registry