Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Hanqing Liu
Date created: 2014-09-27 11:00:00
Date modified: 2015-05-08 01:10:25
RBS-gamma protein-beta protein-exonuclease
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1433005_sequence (Version 1) |
Description
This part is constructed for improve BBa_K1223012, pKD46 functional unit.Lambda red & pKD46 brief introduction.
BBa_K1223012 is not conform to any RFC cause there are three restrict enzyme sites of EcoRI and PstI. As for improve the compatibility of this part and make these useful modules be used in future assemblies, we remove these restrict enzyme sites by synonymous mutations
Notes
NoSource
No| Sequence Annotation | Location | Component / Role(s) |
| RBS GAM-CDS Beta-CDS EXO-CDS T to G(removing Pst1 site) G to A(removing Pst1 site) A to G(removing EcoR1 site) | 1,19 20,436 442,1227 1224,1904 996,996 1247,1247 1502,1502 | feature/rbs ribosome_entry_site feature/cds CDS CDS feature/cds CDS feature/cds feature/s_mutation point_mutation feature/s_mutation point_mutation point_mutation feature/s_mutation |