BBa_K1433006

BBa_K1433006 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1433006

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Hanqing Liu

Date created: 2014-09-27 11:00:00

Date modified: 2015-05-08 01:10:25

B0031-gp35-Terminator

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K1433006_sequence (Version 1)

Description

Bxb1 gp35 is a serine integrase in Mycobacterium phage Bxb1. This intergrase along can typically catalyzes site-specific recombination between attB and attP, the attachment sites on the phage chromosome and host chromosome. This recombination result in reverse of the sequence between attB and attP, then form new sites attL and attR. This revert can restore by appropriately controlling the conditional heterologous expression of integrase and an excisionase in Bxb1 named gp47.
This part is composed of 3 elements.
1. RBS: BBa_B0031, a weak RBS.

2. gp35: a serine integrase in Mycobacterium phage Bxb1.
3. terminator: BBa_B0015, a strong double terminator.

Notes

No

Source

No

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
B0031	1,14	ribosome_entry_site, feature/rbs
BBa_K907000 Bxb1 DNA integrase，gp35	15,1517	CDS, feature/cds
BBa_B0015 Terminator	1518,1646	feature/stem_loop, stem_loop
A to G	15,15	feature/s_mutation, point_mutation

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1433006/1

Member of these Collections

• iGEM Parts Registry