BBa_K1433007

BBa_K1433007 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1433007
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Hanqing Liu
Date created: 2014-09-27 11:00:00
Date modified: 2015-05-08 01:10:25

B0034-gp35-Terminator



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_K1433007_sequence (Version 1)

Description

Bxb1 gp35 is a serine integrase in Mycobacterium phage Bxb1. This intergrase along can typically catalyzes site-specific recombination between attB and attP, the attachment sites on the phage chromosome and host chromosome. This recombination result in reverse of the sequence between attB and attP, then form new sites attL and attR. This revert can restore by appropriately controlling the conditional heterologous expression of integrase and an excisionase in Bxb1 named gp47.
This part is composed of 3 elements.
1. RBS: BBa_B0034, a strong RBS.

2. gp35: a serine integrase in Mycobacterium phage Bxb1.
3. terminator: BBa_B0015, a strong double terminator.

Notes

No

Source

No

Sequence Annotation Location Component / Role(s)
B0034
BBa_K907000 Bxb1 DNA integrase,gp35
BBa_B0015 Terminator
A to G
1,12
13,1515
1516,1644
13,13
ribosome_entry_site feature/rbs
feature/cds CDS
feature/stem_loop stem_loop
feature/s_mutation point_mutation
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/myers
 
synbiohub#ownedBy
user/james
 
synbiohub#topLevel
BBa_K1433007/1