Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Hanqing Liu
Date created: 2014-09-27 11:00:00
Date modified: 2015-05-08 01:10:25
6N-gp35-Terminator
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1433008_sequence (Version 1) |
Description
Bxb1 gp35 is a serine integrase in Mycobacterium phage Bxb1. This intergrase along can typically catalyzes site-specific recombination between attB and attP, the attachment sites on the phage chromosome and host chromosome. This recombination result in reverse of the sequence between attB and attP, then form new sites attL and attR. This revert can restore by appropriately controlling the conditional heterologous expression of integrase and an excisionase in Bxb1 named gp47.This part is composed of 3 elements.
1. RBS: 6N, a suitable RBS in paper.
2. gp35: a serine integrase in Mycobacterium phage Bxb1.
3. terminator: BBa_B0015, a strong double terminator.
Notes
NoSource
No| Sequence Annotation | Location | Component / Role(s) |
| 6N BBa_K907000 Bxb1 DNA integrase，gp35 BBa_B0015 Terminator A to G | 1,25 26,1528 1529,1657 26,26 | feature/rbs ribosome_entry_site CDS feature/cds stem_loop feature/stem_loop feature/s_mutation point_mutation |