BBa_K1441008

BBa_K1441008 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1441008

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Shivani Shah

Date created: 2014-10-05 11:00:00

Date modified: 2015-05-08 01:10:26

PGAPz alpha A + RFP

Details

• Types: DnaRegion
• Roles: Plasmid; plasmid
• Sequences: BBa_K1441008_sequence (Version 1)

Description

We started PGAPz alpha A with modified MCS and ligated it with RFP (iGEM registry parts) after digesting both with ECORI and PstI.

Notes

N/A

Source

RFP was originally extracted from iGEM kit part registry by using right forward and reverse primer for RFP with PCR.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
TTTTTGTAGAAATGTCTTGGTGTCCTCGTCCAATCAGGTAGCCATCTCTGAAATATCTGGCTCCGTTGCAACTCCGAACGACCTGCTGGCAACGTAAAATTCTCCGGGGTAAAACTTAAATGTGGAGTAATGGAACCAGAAACGTCTCTTCCCTTCTCTCTCCTTCCACCGCCCGTTACCGTCCCTAGGAAATTTTACTC	7,483	promoter, feature/promoter
ATGAGATTTCCTTCAATTTTTACTGCTGTTTTATTCGCAGCATCCTCCGCATTAGCTGCTCCAGTCAACACTACAACAGAAGATGAAACGGCACAAATTCCGGCTGAAGCTGTCATCGGTTACTCAGATTTAGAAGGGGATTTCGATGTTGCTGTTTTGCCATTTTCCAACAGCACAAATAACGGGTTATTGTTTATAAA	493,759	engineered_region, feature/BioBrick
tttacactttatgcttccggctcgtatgttg	918,948	feature/promoter, promoter
atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagta	1008,1685	feature/BioBrick, engineered_region
GAACAAAAACTCATCTCAGAAGAGGATCTG	1883,1912	tag, feature/tag
CATCATCATCATCATCAT	1928,1945	feature/tag, tag

Other Properties

• igem#experience: Works
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1441008/1

Member of these Collections

• iGEM Parts Registry