BBa_K1442100

BBa_K1442100 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1442100
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Waqar Yousaf
Date created: 2014-10-05 11:00:00
Date modified: 2015-05-08 01:10:26

RdRP



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K1442100_sequence (Version 1)

Description

This is RdRP, or RNA dependent RNA polymerase. That is, it produces a protein which can bind to a single-strand RNA fragment via an IRES (internal ribosome entry site), read the RNA, and produce from it the same RNA fragment in the opposite sense. This was fundamental to our 'Replicon' project, because it allows the RNA (introduced via viral chassis in the theoretical or transformation in the practical) to self-replicate within a cell.

Notes

It was necessary to remove a PstI binding site which invalidated this part as permissible for iGEM's RFC25 standard.

Source

HMCV?

Sequence Annotation Location Component / Role(s)
TCT to TCC (serine preserved)
901,903
feature/s_mutation point_mutation
igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1442100/1