Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Dan Goss
Date created: 2014-10-05 11:00:00
Date modified: 2015-05-08 01:10:26
RdRP Mutant
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1442101_sequence (Version 1) |
Description
To test the RdRP against a control, we used a deliberately mutated version of itself. To mutate it, we used the restriction enzyme BgLII (code: agatct). This was not thought through but arbitrarily chosen, and resulted in a shift of frame and the introduction of a stop codon (tga).Notes
Principally, our goal with this part was to create a non-functioning version of our RdRP which we could test it against, to ensure it was the RdRP proper that was having the desired effect of replicating our RNA.Source
The source from which this mutant is derived is our RdRP proper, part BBa_K1442100.| Sequence Annotation | Location | Component / Role(s) |
| Stop Codon TGA Untranslated Region | 517,519 517,868 | feature/stop stop_codon feature/misc sequence_feature |