BBa_K1444007

BBa_K1444007 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1444007

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Dan Ziemianowicz

Date created: 2014-10-07 11:00:00

Date modified: 2015-05-08 01:10:27

Composite promoter and weak B. subtilis RBS - C2P22

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K1444007_sequence (Version 1)

Description

A composite part consisting of the constitutive promoter Pveg (BBa_K823003), followed by the repressible promoter C1P22(BBa_R0053),and ending with the weak RBS from B. subtilis (BBa_K780002).

This composite part was intended to control the expression of a downstream reporter, regulated by the expression of the C2P22 repressor protein. The constitutive promoter Pveg allows reliable expression when the repressible promoter is not repressed.

Notes

No interference with endogenous cell signalling.

Source

B. subtilis

Components

Access	Instance	Definition
public	BBa_K823003	BBa_K823003
public	BBa_R0053	cII p22
public	BBa_K780002	R37

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K823003	1,237	BBa_K823003
BBa_R0053	246,299	cII p22
BBa_K780002	308,323	R37

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1444007/1

Member of these Collections

• iGEM Parts Registry